Jacobson Ken, Rajfur Zenon, Vitriol Eric, Hahn Klaus
Department of Cell and Developmental Biology, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599-7090, USA.
Trends Cell Biol. 2008 Sep;18(9):443-50. doi: 10.1016/j.tcb.2008.07.001. Epub 2008 Aug 14.
Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.
生色团辅助激光失活(CALI)是一种技术,通过该技术,经工程改造的蛋白质和染料分子在吸收光后会产生大量活性氧,从而以空间和时间限定的方式干扰生物系统。CALI是传统遗传和药理学操作的重要补充。在本综述中,我们研究了CALI在细胞生物学中的应用,并讨论了介导这种强大技术的潜在光化学机制。