Gomi Yasuyuki, Ozaki Takao, Nishimura Naoko, Narita Atsushi, Suzuki Michio, Ahn Jaekun, Watanabe Naoko, Koyama Norio, Ushida Hajime, Yasuda Naoko, Nakane Kazumasa, Funahashi Keiji, Fuke Isao, Takamizawa Akihisa, Ishikawa Toyokazu, Yamanishi Koichi, Takahashi Michiaki
Kanonji Institute, Research Foundation for Microbial Diseases of Osaka University, 2-9-41, Yahata-cho, Kanonji, Kagawa, Japan.
Vaccine. 2008 Oct 16;26(44):5627-32. doi: 10.1016/j.vaccine.2008.07.069. Epub 2008 Aug 14.
A live attenuated varicella vaccine, the Oka vaccine strain (vOka), is routinely administered to children in Japan and other countries, including the United States. vOka consists of a mixture of genotypically distinct variants, but little is known about the growth potential of each variants in vivo. We isolated varicella-zoster virus (VZV) DNA sequences from the peripheral blood mononuclear cells (PBMCs) of asymptomatic healthy children immunized with the Oka varicella vaccine. VZV gene 62 DNA fragments were detected in 5 of 166 (3.0%) PBMC samples by nested PCR within 5 weeks of the vaccination. Sequence analysis of VZV DNA from these five PBMC samples indicated that multiple viral clones in the vaccine could infect vaccinees and replicate in vivo. We also provide evidence that a nonsynonymous substitution at position 105356 may affect viral replication in vivo.
一种减毒活水痘疫苗，即Oka疫苗株（vOka），在日本及包括美国在内的其他国家常规接种于儿童。vOka由基因上不同的变体混合物组成，但对于每种变体在体内的生长潜力了解甚少。我们从接种Oka水痘疫苗的无症状健康儿童的外周血单核细胞（PBMC）中分离出水痘带状疱疹病毒（VZV）DNA序列。在接种疫苗后5周内，通过巢式PCR在166份PBMC样本中的5份（3.0%）中检测到VZV基因62 DNA片段。对这5份PBMC样本中的VZV DNA进行序列分析表明，疫苗中的多个病毒克隆可感染接种者并在体内复制。我们还提供了证据表明，第105356位的非同义替换可能影响病毒在体内的复制。
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