铜绿假单胞菌中青霉素结合蛋白2与可溶性溶菌转糖基酶B1的相互作用。
Interaction of penicillin-binding protein 2 with soluble lytic transglycosylase B1 in Pseudomonas aeruginosa.
作者信息
Legaree Blaine A, Clarke Anthony J
机构信息
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
出版信息
J Bacteriol. 2008 Oct;190(20):6922-6. doi: 10.1128/JB.00934-08. Epub 2008 Aug 15.
Abstract
Soluble lytic transglycosylase B1 from Pseudomonas aeruginosa was coupled to Sepharose and used to immobilize interaction partners from membrane protein extracts. Penicillin-binding protein 2 (PBP2) was identified as a binding partner, suggesting that the two proteins function together in the biosynthesis of peptidoglycan. By use of an engineered truncated derivative, the N-terminal module of PBP2 was found to confer the binding properties.
摘要
来自铜绿假单胞菌的可溶性溶菌转糖基酶B1与琼脂糖凝胶偶联，并用于固定膜蛋白提取物中的相互作用伙伴。青霉素结合蛋白2（PBP2）被鉴定为结合伙伴，这表明这两种蛋白质在肽聚糖的生物合成中共同发挥作用。通过使用工程化的截短衍生物，发现PBP2的N端模块赋予了结合特性。
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