Akashi Tomoyuki, Shigematsu Hirokazu, Hamamoto Yoshiyuki, Iwasaki Hiromi, Yatoh Shigeru, Bonner-Weir Susan, Akashi Koichi, Weir Gordon C
Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.
Diabetes Metab Res Rev. 2008 Oct;24(7):585-90. doi: 10.1002/dmrr.884.
In this study, we carried out bone marrow and foetal liver cell transplantation to determine if these cells could differentiate into pancreatic beta-cells or promote regeneration.
To exclude an artificial or immunological influence for induction of diabetes to recipients, Akita mice, which develop diabetes spontaneously,were used. In addition, we used mice harbouring the transgenic green fluorescent protein (GFP) reporter for insulin 1 gene as donors to mark donor-derived beta-cells.
All transplanted Akita mice after intravenous injection showed full donor chimerism in peripheral blood analysis. Their diabetic state represented by blood glucose levels did not change after transplantation. In spite of examination of more than 200 islets in each group, we could not find GFP-positive cells in any of the recipients.
Bone marrow cells or foetal liver cells do not differentiate to new pancreatic beta-cells or promote regeneration in Akita mice, a non-chemical or non-immune model of diabetes.
在本研究中，我们进行了骨髓和胎肝细胞移植，以确定这些细胞是否能够分化为胰腺β细胞或促进其再生。
为排除对受体诱导糖尿病的人为或免疫影响，使用了自发发生糖尿病的秋田小鼠。此外，我们使用携带胰岛素1基因转基因绿色荧光蛋白（GFP）报告基因的小鼠作为供体，以标记供体来源的β细胞。
静脉注射后，所有移植的秋田小鼠在外周血分析中均显示出完全的供体嵌合现象。移植后，以血糖水平表示的糖尿病状态没有改变。尽管每组检查了200多个胰岛，但在任何受体中均未发现GFP阳性细胞。
在秋田小鼠（一种非化学或非免疫性糖尿病模型）中，骨髓细胞或胎肝细胞不会分化为新的胰腺β细胞或促进其再生。
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