氨基端甲状旁腺激素片段[PTH(1-34)]对体外培养的牛甲状旁腺原代细胞甲状旁腺激素分泌的直接抑制作用。

Direct inhibitory effect of amino-terminal parathyroid hormone fragment [PTH(1-34)] on PTH secretion from bovine parathyroid primary cultured cells in vitro.

作者信息

Fujimi T, Baba H, Fukase M, Fujita T

机构信息

Department of Medicine, Kobe University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Aug 15;178(3):953-8. doi: 10.1016/0006-291x(91)90984-f.

DOI:10.1016/0006-291x(91)90984-f

PMID:1872875

Abstract

We have examined the possibility of direct inhibitory effect of PTH(1-34) on PTH secretion in bovine parathyroid cells. As low as 10(-12) M PTH(1-34) completely inhibited low calcium (0.5 mM Ca2+)-stimulated PTH secretion by these cells. In the presence of 1.25 mM Ca2+, 10(-12) M PTH(1-34) inhibited PTH secretion by about 14.3% of the basal value, while 10(-11) M or higher concentration of PTH(1-34) showed potent inhibitory effects equivalent to the inhibitory action of high calcium concentration (2.5 mM Ca2+) on PTH secretion. At 2.5 mM Ca2+, as much as 10(-9) M PTH(1-34) failed to inhibit PTH secretion further. These results suggest that PTH(1-34) might directly, not via calcium concentration, inhibit PTH secretion by parathyroid cells and that a cooperative mechanism could exist between calcium and PTH(1-34) to inhibit PTH secretion.

摘要

我们研究了甲状旁腺激素（1-34）[PTH(1-34)]对牛甲状旁腺细胞甲状旁腺激素分泌的直接抑制作用的可能性。低至10^(-12) M的PTH(1-34)就能完全抑制这些细胞在低钙（0.5 mM Ca2+）刺激下的甲状旁腺激素分泌。在1.25 mM Ca2+存在的情况下，10^(-12) M的PTH(1-34)可使甲状旁腺激素分泌抑制至基础值的约14.3%，而10^(-11) M或更高浓度的PTH(1-34)则显示出与高钙浓度（2.5 mM Ca2+）对甲状旁腺激素分泌的抑制作用相当的强效抑制作用。在2.5 mM Ca2+时，高达10^(-9) M的PTH(1-34)也未能进一步抑制甲状旁腺激素分泌。这些结果表明，PTH(1-34)可能直接而非通过钙浓度抑制甲状旁腺细胞的甲状旁腺激素分泌，并且钙和PTH(1-34)之间可能存在协同机制来抑制甲状旁腺激素分泌。