促卵泡激素和促黄体生成素对体外培养的大鼠卵巢中环磷酸腺苷和环磷酸鸟苷水平的影响。

Effects of follicle stimulating hormone and luteinizing hormone upon cyclic AMP and cyclic GMP levels in rat ovaries in vitro.

作者信息

Ratner A

出版信息

Endocrinology. 1976 Dec;99(6):1496-500. doi: 10.1210/endo-99-6-1496.

Abstract

In vitro effects of FSH and LH on adenosine 3',5'-cyclic monophosphate (cyclic AMP) and guanosine 3',5'-cyclic monophosphate (cyclic GMP) levels in rat ovarian tissue were studied. Ovaries from immature rats treated with either pregnant mare's serum (PMS) or PMS and human chorionic gonadotropin (hCG) were incubated at 37 C for 30 min in defined medium 199 containing aminophylline (0.1 mg/ml). Increasing concentrations of LH (0.4-10.0 mug) when added to the medium containing ovaries with predominantly follicular tissue (PMS-treated) produced progressive increases in cyclic AMP (29-300%) and concomitant decreases in cyclic GMP (41-70%). FSH (4-100 mug) added to the medium produced comparable changes in nucleotide levels: cyclic AMP increased 52-390% and cyclic GMP decreased 45-80%. Ovaries with predominantly luteal tissue (PMS- and hCG-treated) responded differently to the same experimental conditions. LH produced a much greater increase in tissue cyclic AMP levels (209-1280%), whereas FSH produced comparable increases in cyclic AMP as seen with follicular tissue (103-570%). Neither gonadotropin caused any changes in cyclic GMP levels in luteinized ovaries. These results indicate that rat ovarian cyclic GMP is regulated by gonadotropins and the differences in the direction of nucleotide response to gonadotropins suggest divergent roles for cyclic AMP and cyclic GMP in ovarian function.

摘要

研究了促卵泡激素（FSH）和促黄体生成素（LH）对大鼠卵巢组织中3',5'-环磷酸腺苷（环磷酸腺苷，cyclic AMP）和3',5'-环磷酸鸟苷（环磷酸鸟苷，cyclic GMP）水平的体外作用。将用孕马血清（PMS）或PMS与人绒毛膜促性腺激素（hCG）处理的未成熟大鼠的卵巢，在含有氨茶碱（0.1 mg/ml）的限定培养基199中于37℃孵育30分钟。当向含有主要为卵泡组织的卵巢（经PMS处理）的培养基中添加浓度递增的LH（0.4 - 10.0微克）时，环磷酸腺苷水平逐渐升高（29% - 300%），同时环磷酸鸟苷水平相应降低（41% - 70%）。向培养基中添加FSH（4 - 100微克）时，核苷酸水平出现类似变化：环磷酸腺苷升高52% - 390%，环磷酸鸟苷降低45% - 80%。主要为黄体组织的卵巢（经PMS和hCG处理）对相同实验条件的反应不同。LH使组织中环磷酸腺苷水平升高幅度更大（达209% - 1280%），而FSH使环磷酸腺苷升高幅度与卵泡组织所见相当（103% - 570%）。两种促性腺激素均未引起黄体化卵巢中环磷酸鸟苷水平的任何变化。这些结果表明，大鼠卵巢中的环磷酸鸟苷受促性腺激素调节，且对促性腺激素核苷酸反应方向的差异表明环磷酸腺苷和环磷酸鸟苷在卵巢功能中具有不同作用。