采用气相色谱-质谱联用技术检测牛尿液中非法使用的乙炔雌二醇。

Detection of the illegal use of ethinylestradiol in cattle urine by gas chromatography-mass spectrometry.

作者信息

Daeseleire E, De Guesquière A, Van Peteghem C

机构信息

Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Belgium.

出版信息

J Chromatogr. 1991 Apr 5;564(2):469-75. doi: 10.1016/0378-4347(91)80515-e.

DOI:10.1016/0378-4347(91)80515-e

PMID:1874851

Abstract

A method for the detection of ethinylestradiol in cattle urine is described, based on enzymic hydrolysis of the sample, clean-up by means of disposable octadecyl and amino solid-phase extraction columns, fractionation by reversed-phase high-performance liquid chromatography, and detection by gas chromatography-mass spectrometry (selected-ion monitoring). Identification is based on both gas chromatographic and mass spectrometric data. The method has been tested on urine samples for a collaborative study and all the results found were correct.

摘要

本文描述了一种检测牛尿液中炔雌醇的方法，该方法基于对样品进行酶水解、通过一次性十八烷基和氨基固相萃取柱进行净化、利用反相高效液相色谱进行分离以及采用气相色谱 - 质谱联用（选择离子监测）进行检测。鉴定基于气相色谱和质谱数据。该方法已在尿液样本上进行了协同研究测试，所有检测结果均正确。