胶质母细胞瘤细胞中金丝桃素与内质网和高尔基体的亚细胞共定位。
Subcellular colocalization of hypericin with respect to endoplasmic reticulum and Golgi apparatus in glioblastoma cells.
作者信息
Ritz Rainer, Roser Florian, Radomski Norbert, Strauss Wolfgang S L, Tatagiba Marcos, Gharabaghi Alireza
机构信息
Department of Neurosurgery, Eberhard-Karls-Universität Tübingen, Hoppe-Seyler-Strasse 3, 72076 Tübingen, Germany.
出版信息
Anticancer Res. 2008 Jul-Aug;28(4B):2033-8.
BACKGROUND
To improve the poor prognosis of patients suffering from malignant glioma, hypericin (HYP)-based photodynamic therapy might be a promising approach. Intracellular localization of HYP was investigated by quantitative colocalization analysis with respect to endoplasmic reticulum (ER) and Golgi apparatus (GA) by double staining experiments with fluorescence microscopy.
MATERIALS AND METHODS
U373 MG glioblastoma cells were stained with HYP and costainings were applied for specific organelle markers for ER and GA.
RESULTS
In cells double-stained with HYP and ER-Tracker, 57% of HYP signals were found within the ER and 52% of the ER compartment showed HYP signals. The colocalization fraction of HYP found in the GA was 36% and 46% of the GA showed HYP signals.
CONCLUSION
In glioblastoma cells, a considerable fraction of HYP is localized in the ER; in addition, a significant amount of the photosensitizer shows colocalization with the GA.
背景
为改善恶性胶质瘤患者的不良预后，基于金丝桃素（HYP）的光动力疗法可能是一种有前景的方法。通过荧光显微镜双染实验，采用定量共定位分析研究了HYP相对于内质网（ER）和高尔基体（GA）的细胞内定位。
材料与方法
用HYP对U373 MG胶质母细胞瘤细胞进行染色，并对ER和GA的特定细胞器标记物进行共染色。
结果
在HYP与ER-Tracker双染的细胞中，57%的HYP信号在内质网内被发现，52%的内质网区域显示有HYP信号。在高尔基体中发现的HYP共定位分数为36%，46%的高尔基体显示有HYP信号。
结论
在胶质母细胞瘤细胞中，相当一部分HYP定位于内质网；此外，大量的光敏剂与高尔基体共定位。
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