成纤维细胞生长因子受体2型的恢复增强了激素难治性人前列腺癌PC-3细胞的放射敏感性。
Restoration of FGF receptor type 2 enhances radiosensitivity of hormone-refractory human prostate carcinoma PC-3 cells.
作者信息
Matsubara Akio, Teishima Jun, Mirkhat Suichinov, Yasumoto Hiroaki, Mochizuki Hideki, Seki Mitsuhiro, Mutaguchi Kazuaki, Mckeehan Wallace L, Usui Tsuguru
机构信息
Department of Urology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
出版信息
Anticancer Res. 2008 Jul-Aug;28(4B):2141-6.
BACKGROUND
This study was undertaken to investigate the radiosensitizing effects of fibroblast growth factor receptor 2IIIb (FGFR2IIIb) in androgen-independent human prostate carcinoma PC-3 cells devoid of normally resident epithelial cell FGFR2IIIb.
MATERIALS AND METHODS
A clonal line of PC-3 cells expressing FGFR2IIIb was established by stable transfection. Clonogenic cell survival, apoptosis and cell cycle distribution with and without gamma-irradiation were then compared between FGFR2IIIb-expressing PC-3 cells and control cells mock-transfected with vector alone.
RESULTS
Gamma-irradiation resulted in an increase of clonogenic cell death concurrent with enhanced apoptosis and cell cycle arrest in the G2/M-phase in both transfected and untransfected cells. A quantitative analysis of all three parameters indicated that cells expressing FGFR2IIIb were significantly more sensitive to irradiation than control cells.
CONCLUSION
These results indicate that restoration of FGFR2IIIb to PC-3 cells enhances their sensitivity to irradiation through acceleration of apoptosis and cell cycle arrest.
背景
本研究旨在探讨成纤维细胞生长因子受体2IIIb（FGFR2IIIb）对缺乏正常驻留上皮细胞FGFR2IIIb的雄激素非依赖性人前列腺癌PC-3细胞的放射增敏作用。
材料与方法
通过稳定转染建立表达FGFR2IIIb的PC-3细胞克隆系。然后比较表达FGFR2IIIb的PC-3细胞与仅用载体进行模拟转染的对照细胞在有无γ射线照射情况下的克隆形成细胞存活率、凋亡情况和细胞周期分布。
结果
γ射线照射导致转染和未转染细胞的克隆形成细胞死亡增加，同时凋亡增强且细胞周期停滞于G2/M期。对所有三个参数的定量分析表明，表达FGFR2IIIb的细胞对辐射的敏感性明显高于对照细胞。
结论
这些结果表明，将FGFR2IIIb恢复至PC-3细胞可通过加速凋亡和细胞周期停滞来增强其对辐射的敏感性。
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