Zhu Senlin, Xia Harry Hua-Xiang, Yang Yi, Ma Juan, Chen Minhu, Hu Pinjin, Gu Qing, Liang Yingjie, Lin Hanliang, Wong Benjamin C Y
Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Dig Dis Sci. 2009 May;54(5):996-1002. doi: 10.1007/s10620-008-0459-8. Epub 2008 Aug 27.
This study was to determine whether gastric expression of homeoproteins is altered in Helicobacter pylori infection, incisural antralisation, and intestinal metaplasia (IM).
Gastric biopsy specimens were taken from 98 patients with non-ulcer dyspepsia for the detection of H. pylori infection; histological examinations; immunohistochemical staining of CDX2, PDX1, PAX6, and NKX6.1.
Of the patients, 38 were positive for H. pylori infection, 44 had antral-type mucosa at the incisura, and 22 had IM in the stomach. At the incisura, the expression of PDX1, NKX6.1, and PAX6 in cytoplasm compartment was down-regulated in antral-type mucosa compared with that in the transitional- or body-type mucosa (all P<0.01). The expression of PDX1, PAX6, and NKX6.1 in cytoplasm at the incisura was down-regulated in H. pylori-infected patients compared with that in those without H. pylori infection (all P<0.01). CDX2 expression in whole stomach was up-regulated, but PDX1 expression at the incisura was down-regulated in patients with IM compared with that in those without IM (all P<0.01).
Gastric expression of PDX1, PAX 6, and NKX6.1 is down-regulated in H. pylori infection and incisural antralisation. CDX2 is up-regulated but PDX1 is down-regulated in the presence of IM.
本研究旨在确定在幽门螺杆菌感染、胃切迹窦化及肠化生（IM）过程中，同源异形蛋白的胃表达是否发生改变。
从98例非溃疡性消化不良患者中获取胃活检标本，用于检测幽门螺杆菌感染、组织学检查以及CDX2、PDX1、PAX6和NKX6.1的免疫组织化学染色。
患者中，38例幽门螺杆菌感染阳性，44例胃切迹处为窦型黏膜，22例胃内有肠化生。在胃切迹处，与移行型或胃体型黏膜相比，窦型黏膜中PDX1、NKX6.1和PAX6在细胞质区的表达下调（均P<0.01）。与未感染幽门螺杆菌的患者相比，感染幽门螺杆菌的患者胃切迹处细胞质中PDX1、PAX6和NKX6.1的表达下调（均P<0.01）。与无肠化生的患者相比，肠化生患者全胃CDX2表达上调，但胃切迹处PDX1表达下调（均P<0.01）。
在幽门螺杆菌感染和胃切迹窦化过程中，PDX1、PAX6和NKX6.1的胃表达下调。在存在肠化生时，CDX2上调但PDX1下调。
