感染致病性和非致病性锥虫的大鼠的比较组织化学研究。
Comparative histochemical studies of rats infected with a pathogenic and nonpathogenic trypanosome.
作者信息
Guerrero V, Durham C, Lee C M
机构信息
Department of Food Sciences, University of the District of Columbia, Washington.
出版信息
J Natl Med Assoc. 1991 May;83(5):441-56.
Histochemical variations in tissues from rats inoculated with Trypanosoma lewisi and Trypanosoma rhodesiense were investigated. During peak parasitemia, the liver of rats inoculated with T lewisi showed increased glycogen distribution. However, glycogen depletion was noted in the liver and spleen of animals inoculated with living cells of T rhodesiense. Depletion was very apparent from day 4 to day 10. Throughout the period of observation, only a small amount of lipid infiltration occurred in tissues from animals inoculated with both organisms. Protein tests revealed a normal distribution of protein in tissues. Sections of the liver from rats inoculated with T lewisi showed strong alkaline phosphatase activity on days 7, 10, and 13. Alkaline phosphatase activity for T rhodesiense-infected animals was positive for days 4, 7, and 10. Strong positive reactions for acid phosphatase were observed on days 10 and 13 for some tissues (liver, spleen, and kidney) from rats inoculated with T lewisi. On days 4, 7, and 10, intense staining reactions also were observed for livers and spleens of animals inoculated with T rhodesiense. Regardless of tissues observed, histochemical variations were not observed in animals inoculated with the derivatives (ie, metabolic products and homogenates) of T lewisi and T rhodesiense.
研究了接种杜氏利什曼原虫和罗得西亚锥虫的大鼠组织中的组织化学变化。在寄生虫血症高峰期，接种杜氏利什曼原虫的大鼠肝脏中糖原分布增加。然而，在接种罗得西亚锥虫活细胞的动物的肝脏和脾脏中发现糖原耗竭。从第4天到第10天，糖原耗竭非常明显。在整个观察期内，接种两种寄生虫的动物组织中仅出现少量脂质浸润。蛋白质检测显示组织中蛋白质分布正常。接种杜氏利什曼原虫的大鼠肝脏切片在第7、10和13天显示出强烈的碱性磷酸酶活性。感染罗得西亚锥虫的动物在第4、7和10天碱性磷酸酶活性呈阳性。接种杜氏利什曼原虫的大鼠的一些组织（肝脏、脾脏和肾脏）在第10和13天观察到酸性磷酸酶的强阳性反应。在第4、7和10天，接种罗得西亚锥虫的动物的肝脏和脾脏也观察到强烈的染色反应。无论观察何种组织，接种杜氏利什曼原虫和罗得西亚锥虫衍生物（即代谢产物和匀浆）的动物均未观察到组织化学变化。
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