马兜铃酸体外产生一氧化氮的作用研究。

Study on the nitric oxide generating effects from aristolochic acid in vitro.

作者信息

Xu Qin, Ma Chaomei, Wang Wei, Fu Xiang, Tang Chunyan, Hattori Masao

机构信息

Institute of Nature Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

出版信息

Eur J Mass Spectrom (Chichester). 2008;14(4):231-7. doi: 10.1255/ejms.932.

DOI:10.1255/ejms.932

PMID:18756021

Abstract

In this study, an in vitro nitric oxide (NO)-assay system based on the Griess reaction was used to investigate the (NO)-generating effects of aristolochic acid (AA) for the first time. AA was separated into its different components, aristolochic acid I (AAI) and aristolochic acid II (AAII), by preparative HPLC. AAI and AAII were incubated with human intestine bacteria (HIB) or rat intestine bacteria (RIB). A NO mixture generated from AAI and AAII by intestinal bacteria was observed and denitroso metabolites of AAI or AAII were detected in vitro by liquid chromatography/tandem mass spectrometry. Therefore, NO generation might be closely related to the metabolic process of AA in vitro. It suggested that one possible mechanism for the toxicity of AA may be due to the generation of NO from these compounds by intestinal bacteria.

摘要

在本研究中，首次使用基于格里斯反应的体外一氧化氮（NO）检测系统来研究马兜铃酸（AA）的NO生成效应。通过制备型高效液相色谱法将AA分离成其不同成分，即马兜铃酸I（AAI）和马兜铃酸II（AAII）。将AAI和AAII与人肠道细菌（HIB）或大鼠肠道细菌（RIB）一起孵育。观察到由肠道细菌从AAI和AAII产生的NO混合物，并通过液相色谱/串联质谱法在体外检测到AAI或AAII的脱亚硝基代谢物。因此，NO生成可能与体外AA的代谢过程密切相关。这表明AA毒性的一种可能机制可能是由于肠道细菌从这些化合物中生成NO。