Huang S J, Cheng L, Lewin K J, Fu Y S
Department of Pathology, UCLA School of Medicine.
Pathol Res Pract. 1991 May;187(4):487-95. doi: 10.1016/S0344-0338(11)80012-9.
Endometrial hyperplasias and some endometrial carcinomas arise in a setting of estrogen excess. Steroid hormones interact with cells via specific receptors; assessing receptor levels may indicate a tissue's potential for interaction with that hormone. To examine estrogen receptor (ER) levels in endometrial hyperplasia, endometrial carcinoma, and physiologically cycling endometrium, an immunohistochemical technique utilizing a monoclonal anti-estrophilin (estrogen receptor) antibody was applied to formalin-fixed, paraffin-embedded tissue. In complex hyperplasia and grade I adenocarcinoma, the mean percentages of epithelial cells demonstrating nuclear staining for ER was mildly decreased compared to proliferative endometrium. A trend was noted toward less ER staining in atypical hyperplasia compared to non-atypical complex hyperplasia. ER varied with physiologic cycling of the endometrium. ER was also present in atrophic endometrium, myometrium, adenomyosis, and leiomyomata. Immunohistochemistry permits localization of ER and is a useful technique in ER assessment of endometrial hyperplasias and carcinomas.
子宫内膜增生和一些子宫内膜癌发生于雌激素过多的情况下。类固醇激素通过特定受体与细胞相互作用；评估受体水平可能表明组织与该激素相互作用的潜力。为了检测子宫内膜增生、子宫内膜癌和生理性周期性子宫内膜中的雌激素受体（ER）水平，将一种利用单克隆抗雌激素受体抗体的免疫组织化学技术应用于福尔马林固定、石蜡包埋的组织。在复杂性增生和I级腺癌中，与增殖期子宫内膜相比，显示ER核染色的上皮细胞平均百分比轻度降低。与非非典型性复杂性增生相比，非典型增生中ER染色有减少的趋势。ER随子宫内膜的生理周期而变化。ER也存在于萎缩性子宫内膜、子宫肌层、子宫腺肌病和平滑肌瘤中。免疫组织化学可对ER进行定位，是评估子宫内膜增生和癌中ER的有用技术。
