来自宫崎F型普通脱硫弧菌的风味毒素的结晶及初步X射线晶体学研究
Crystallization and preliminary X-ray crystallographic study of flavoredoxin from Desulfovibrio vulgaris Miyazaki F.
作者信息
Ueda Yasufumi, Shibata Naoki, Takeuchi Daisuke, Kitamura Masaya, Higuchi Yoshiki
机构信息
Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):851-3. doi: 10.1107/S1744309108025840. Epub 2008 Aug 20.
Abstract
Flavoredoxin from Desulfovibrio vulgaris Miyazaki F has been overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method with 10%(w/v) PEG 8000, 0.2 M zinc acetate and 100 mM MES pH 6.0. The diffraction pattern of the crystal extended to 1.05 A resolution under cryogenic conditions. The space group was determined to be P3(1)21, with unit-cell parameters a = b = 53.35, c = 116.22 A. Phase determination was carried out by the SAD method using methylmercuric chloride.
摘要
来自普通脱硫弧菌宫崎F株的风味氧化还原蛋白已通过坐滴气相扩散法，在含有10%（w/v）聚乙二醇8000、0.2 M醋酸锌和100 mM MES（pH 6.0）的条件下进行了过量表达、纯化和结晶。该晶体在低温条件下的衍射图谱分辨率达到1.05 Å。确定其空间群为P3(1)21，晶胞参数a = b = 53.35，c = 116.22 Å。使用氯化甲基汞通过单波长反常散射法进行相位测定。
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