Roberts Samantha, Scott June R, Husmann Linda K, Zurawski Christine A
Emory University School of Medicine, Atlanta, Georgia, USA.
Curr Protoc Microbiol. 2006 Sep;Chapter 9:Unit 9D.5. doi: 10.1002/9780471729259.mc09d05s02.
This unit describes procedures for testing virulence of Streptococcus pyogenes in mice. S. pyogenes is an important human pathogen and causes one of the most common childhood diseases. The syndromes that result from S. pyogenes infection are diverse, ranging from mild, superficial throat or skin infection to severe, invasive disea/se that is often lethal. Thus, a greater understanding of the virulence factors of this bacterium and development of modalities to prevent or relieve the infections it causes are important. Since S. pyogenes is a strictly human pathogen (with the exception of a single strain), the value of all animal models is limited. This unit describes a model for long-term throat colonization following the natural route of infection (inhalation), one for pneumonia and systemic dissemination following intratracheal inoculation, and one for systemic dissemination following the more natural route of skin infection. In addition, methods are presented for culturing S. pyogenes from tissues of the infected animal.
本单元介绍了在小鼠中测试化脓性链球菌毒力的程序。化脓性链球菌是一种重要的人类病原体，可引发最常见的儿童疾病之一。由化脓性链球菌感染导致的综合征多种多样，从轻症、浅表性咽喉或皮肤感染到严重的侵袭性疾病，后者往往是致命的。因此，深入了解这种细菌的毒力因子以及开发预防或缓解其所致感染的方法至关重要。由于化脓性链球菌是一种严格的人类病原体（除单一菌株外），所有动物模型的价值都有限。本单元描述了一种通过自然感染途径（吸入）进行长期咽喉定植的模型、一种通过气管内接种引发肺炎和全身播散的模型，以及一种通过更自然的皮肤感染途径进行全身播散的模型。此外，还介绍了从受感染动物组织中培养化脓性链球菌的方法。
