用于掺入2'-5' RNA:3'-5' RNA双链体的锁定S型和锁定N型尿苷单体单元的合成。

Synthesis of locked S-type and locked N-type uridine monomer units for incorporation in 2'-5' RNA:3'-5'RNA duplexes.

作者信息

Gunjal Anita D, Erande Namrata, D'Costa Moneesha, Kumar Vaijayanti A

机构信息

Division of Organic Chemistry, National Chemical Laboratory, Pune, 411008 India.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):191-2. doi: 10.1093/nass/nrn097.

DOI:10.1093/nass/nrn097

PMID:18776318

Abstract

To delineate the structural requirements of 2'-5' RNA:3'-5' RNA duplexes, we report the synthesis of N-type locked, S-type locked and 3'-fluoro-2'-phosphoramidite building blocks. The consequent incorporation of these three novel monomers into 2'-5' linked oligomers and their biophysical implications on the stability of the said duplexes will have explicit importance towards development into therapeutic oligomers. The intrinsic stability of 2'-5' phosphodiester linkage as opposed to 3'-5' linked oligomers will be an added advantage.

摘要

为了阐明2'-5' RNA与3'-5' RNA双链体的结构要求，我们报道了N型锁核酸、S型锁核酸和3'-氟-2'-亚磷酰胺砌块的合成。随后将这三种新型单体掺入2'-5'连接的寡聚物中，以及它们对所述双链体稳定性的生物物理影响，对于开发治疗性寡聚物将具有明确的重要性。与3'-5'连接的寡聚物相比，2'-5'磷酸二酯键的固有稳定性将是一个额外的优势。