为X射线晶体学合成的2'-甲基硒修饰的寡核糖核苷酸。

2'-Methylseleno modified oligoribonucleotides synthesized for X-ray crystallography.

作者信息

Moroder Holger, Puffer Barbara, Micura Ronald

机构信息

Institute of Organic Chemistry, Center for Molecular Biosciences Innsbruck, Leopold Franzens University Innsbruck, Innrain 52a, Innsbruck, Austria.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):315-6. doi: 10.1093/nass/nrn159.

DOI:10.1093/nass/nrn159

PMID:18776380

Abstract

Site-specifically modified 2'-methylseleno RNA represents a valuable derivative for phasing of X-ray crystallographic data. Several successful applications in three-dimensional structure determination of nucleic acids, such as the Diels-Alder ribozyme,(1) have relied on these modifications.(2) Currently, preparation of 2'-methylseleno modified oligoribonucleotides is restricted to the 2'-O-[(triisopropylsilyl)oxy]methyl (TOM)(3,4,5) and 2'-O-tert.-butyldimethylsilyl (TBDMS) RNA synthesis methods. Recently, we have introduced synthetic routes to 2'-methylseleno phosphoramidite building blocks of all four standard nucleosides, adenosine, uridine, guanosine, cytidine, that are tailored for the 2'-O-bis(acetoxyethoxy)methyl (ACE) RNA solid-phase synthesis method.(6).

摘要

位点特异性修饰的2'-甲基硒代RNA是用于X射线晶体学数据相位分析的一种有价值的衍生物。在核酸三维结构测定中的几个成功应用，如狄尔斯-阿尔德核酶，都依赖于这些修饰。目前，2'-甲基硒代修饰的寡核糖核苷酸的制备仅限于2'-O-[(三异丙基甲硅烷基)氧基]甲基（TOM）和2'-O-叔丁基二甲基甲硅烷基（TBDMS）RNA合成方法。最近，我们已经引入了针对2'-O-双（乙酰氧基乙氧基）甲基（ACE）RNA固相合成方法定制的所有四种标准核苷（腺苷、尿苷、鸟苷、胞苷）的2'-甲基硒代亚磷酰胺构建块的合成路线。