苯基吡咯并胞嘧啶在肽核酸中的优异荧光和杂交特性。

Exceptional fluorescence and hybridization properties of a phenylpyrrolocytosine in peptide nucleic acid.

作者信息

Wojciechowski Filip, Hudson Robert H E

机构信息

Department of Chemistry, The University of Western Ontario, London, Ontario, Canada.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):401-2. doi: 10.1093/nass/nrn204.

DOI:10.1093/nass/nrn204

PMID:18776423

Abstract

A phenylpyrrolocytosine derivative possessing bis-(ortho-aminoethoxy) substitution has been designed, synthesized and incorporated into PNA resulting in oligomers that demonstrate increased binding affinity for DNA targets. The phenylpyrrolocytosine is an exceptionally fluorescent modified nucleobase possessing a high quantum yield while the fluorescence intensity displays useful environmental sensitivity.

摘要

一种具有双（邻氨基乙氧基）取代的苯基吡咯并胞嘧啶衍生物已被设计、合成并掺入肽核酸（PNA）中，从而得到了对DNA靶标具有更高结合亲和力的寡聚物。苯基吡咯并胞嘧啶是一种具有高量子产率的异常荧光修饰核碱基，其荧光强度表现出有用的环境敏感性。