Purzycka Katarzyna J, Adamiak Ryszard W
Laboratory of Structural Chemistry of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.
Nucleic Acids Symp Ser (Oxf). 2008(52):511-2. doi: 10.1093/nass/nrn259.
Recently, it has been reported that HIV-1 TAR RNA element releases functionally competent miRNAs upon processing by Dicer enzyme. Here, we extend the analysis of miRNA viral-encoding potential to the TAR RNA of the HIV-2. Using in vitro Dicer cleavages and computer-aided analysis we have found that the 124-mer TAR RNA domain, present at the 5' end of HIV-2 mRNAs, putatively encodes pre-miRNAs. When deduced sequences of the viral-encoded miRNAs were matched against the database of human mRNA 3'-UTRs, it appeared that two miRNA candidates may target a large number of cellular transcripts.
最近，有报道称HIV-1 TAR RNA元件经Dicer酶加工后会释放出具有功能活性的微小RNA（miRNA）。在此，我们将对miRNA病毒编码潜力的分析扩展至HIV-2的TAR RNA。通过体外Dicer切割和计算机辅助分析，我们发现存在于HIV-2 mRNA 5'端的124个核苷酸的TAR RNA结构域可能编码前体miRNA。当将病毒编码的miRNA的推导序列与人类mRNA 3'-非翻译区（UTR）数据库进行比对时，发现有两个miRNA候选序列可能靶向大量细胞转录本。
