具有3'-修饰的寡聚（2'-O-甲基核糖核苷酸）效应物的10-23 DNA酶串联体对结构化RNA的有效切割。

Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3'-modified oligo(2'-O-methylribonucleotide)-effectors.

作者信息

Fokina Alesya, Novopashina Daria, Meschaninova Mariya, Vorobjeva Maria, Zenkova Marina, Francois Jean-Christophe, Venyaminova Alya

机构信息

Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrent'ev ave., Novosibirsk 630090, Russia.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):525-6. doi: 10.1093/nass/nrn266.

DOI:10.1093/nass/nrn266

PMID:18776485

Abstract

Catalytic activity of DNAzymes targeted to IGF-I and MDR1 mRNA can be regulated by the combination of 10-23 DNAzyme with 3'-modified oligo(2'-O-methylribonucleotides) that are favorable as effectors due to their high affinity to RNA and nuclease resistance. We have demonstrated that the DNAzyme constructions designed were able to bind and cleave long structured RNA transcripts effectively under simulated physiological conditions.

摘要

靶向胰岛素样生长因子-I（IGF-I）和多药耐药基因1（MDR1）信使核糖核酸（mRNA）的脱氧核酶（DNAzyme）的催化活性可通过将10-23脱氧核酶与3'-修饰的寡聚（2'-O-甲基核糖核苷酸）相结合来调节，由于它们对RNA具有高亲和力且具有核酸酶抗性，因此作为效应物很有利。我们已经证明，所设计的脱氧核酶构建体能够在模拟生理条件下有效结合并切割长链结构化RNA转录本。