Key Laboratory of Protein Chemistry and Fish Development Biology of National Education Ministry, College of Life Science, Hunan Normal University, Changsha 410081, China.
Fish Physiol Biochem. 2010 Jun;36(2):213-21. doi: 10.1007/s10695-008-9223-4. Epub 2008 Sep 6.
Follicle-stimulating hormone beta subunit (FSHbeta) and luteinizing hormone beta subunit (LHbeta) have been cloned and characterized from the pituitary of grass carp (Ctenopharyngodon idella). The full length of FSHbeta and LHbeta cDNA was 393 bp and 441 bp, with open reading frame encoding proteins of 130 and 146 amino acids, respectively. Phylogenetic analysis of the proteins of FSHbeta and LHbeta showed a high homology with other fishes. Homology analysis also indicated that LHbeta has higher conservation than FSHbeta. The expression analysis of grass carp FSHbeta and LHbeta by RT-PCR suggested that they were only expressed in the pituitary. Real-time quantitative PCR protocols were developed and validated to measure FSHbeta and LHbeta mRNAs during ovarian development. The FSHbeta and LHbeta mRNA level was very low in the pituitaries of early-pubertal fish and significantly increased during the ovulation period. These results suggested that in grass carp the gonadotropins synthesized synchronously in order for asynchronous oogenesis to take place.
已从草鱼（Ctenopharyngodon idella）脑垂体中克隆并鉴定了卵泡刺激素β亚基（FSHβ）和促黄体生成素β亚基（LHβ）。FSHβ 和 LHβ cDNA 的全长分别为 393bp 和 441bp，开放阅读框编码的蛋白质分别为 130 个和 146 个氨基酸。FSHβ 和 LHβ 蛋白的系统发育分析显示与其他鱼类具有高度同源性。同源性分析还表明，LHβ 比 FSHβ 具有更高的保守性。通过 RT-PCR 对草鱼 FSHβ 和 LHβ 的表达分析表明，它们仅在脑垂体中表达。建立并验证了实时定量 PCR 方案，以测量卵巢发育过程中 FSHβ 和 LHβ mRNA 的水平。在早期性成熟鱼的脑垂体中，FSHβ 和 LHβ mRNA 水平非常低，在排卵期间显著增加。这些结果表明，在草鱼中，为了发生异步卵发生，同步合成促性腺激素。
