基于质谱法检测葡萄球菌属蛋白质的方法。
Mass spectrometry-based approaches for the detection of proteins of Staphylococcus species.
作者信息
Ho Yen-Peng, Reddy P Muralidhar
机构信息
Department of Chemistry, National Dong Hwa University, Hualien 97401, Taiwan.
出版信息
Infect Disord Drug Targets. 2008 Sep;8(3):166-82. doi: 10.2174/1871526510808030166.
Mass spectrometry (MS) has become a powerful and popular method to analyze macromolecules from biological systems towards the application of clinical chemistry. Disease markers related to infections can be identified with MS analysis in combination with electrophoresis or chromatographic separations. Further, direct analysis of whole pathogenic bacterial cells (taken directly from a colony) by MS can reveal specific biomarkers that can be used for taxonomy. A brief introduction to the two advanced ionization techniques, electrospray ionization and matrix-assisted laser desorption/ionization, for MS is provided in this review. Sample preparation, separation and MS-related techniques for staphylococcal proteins analysis are summarized. The review is concluded with some current clinical applications of mass spectrometry in the area of biomarker research, vaccine development, diagnosis and strain typing of infectious Staphylococci.
质谱分析法（MS）已成为一种强大且流行的方法，用于分析生物系统中的大分子，以应用于临床化学领域。与感染相关的疾病标志物可通过质谱分析结合电泳或色谱分离来鉴定。此外，通过质谱直接分析整个致病细菌细胞（直接从菌落中获取）可以揭示可用于分类学的特定生物标志物。本文综述了质谱分析法的两种先进电离技术，即电喷雾电离和基质辅助激光解吸/电离。总结了用于葡萄球菌蛋白质分析的样品制备、分离和与质谱相关的技术。本文最后介绍了质谱分析法目前在生物标志物研究、疫苗开发、感染性葡萄球菌的诊断和菌株分型等领域的一些临床应用。
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