Wang Ying, DU Ke-xin, Li Ming, Shi Jing-hong, Xie Wen
Department of Physiology and Pathophysiology, Key Laboratory of Environment and Gene Related to Disease of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Sep;24(9):867-9.
To investigate the expression of caspase 3 in the brain regions related to addiction, learning and memory in mice prenatally exposed to heroin and to ascertain whether postnatal apoptotic mechanism participates in neurobehavioral teratogenicity induced by maternal heroin abuse.
A mouse model was established by administration of diacetylmorphine (heroin, purity 98.5%, product ID No.171206-200614) 10 mg/(kg x d) subcutaneously to pregnant BALB/c mice on embryonic day (E)E8-E18. The offspring were divided into heroin(Her) and saline(Sal) groups according to the maternal treatment. The expression of caspase 3 in prefrontal lobe cortex(PFC), hippocampus(HP) and nucleus accumbens(Acb) was detected by RT-PCR and Western blot on mouse postnatal day(P)14.
The mRNA and protein expression of caspase 3 were significantly increased in the areas of PFC, HP and Acb in Her group compared with Sal group(P < 0.05).
E8-E18 prenatal exposure to heroin can induce apoptosis through caspase 3 activation in brain regions related to addiction, learning and memory, which indicates that apoptotic mechanism may be involved in neurobehavioral teratogenicity by heroin exposure in uterus.
研究产前暴露于海洛因的小鼠成瘾、学习和记忆相关脑区中半胱天冬酶3（caspase 3）的表达，并确定产后凋亡机制是否参与母体海洛因滥用所致的神经行为致畸性。
在胚胎期（E）E8 - E18，对怀孕的BALB/c小鼠皮下注射10 mg/（kg·d）二乙酰吗啡（海洛因，纯度98.5%，产品编号171206 - 200614）建立小鼠模型。根据母体处理情况将子代分为海洛因（Her）组和生理盐水（Sal）组。在小鼠出生后第14天（P14），通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测前额叶皮质（PFC）、海马体（HP）和伏隔核（Acb）中caspase 3的表达。
与Sal组相比，Her组PFC、HP和Acb区域中caspase 3的mRNA和蛋白质表达显著增加（P < 0.05）。
产前E8 - E18暴露于海洛因可通过激活成瘾、学习和记忆相关脑区中的caspase 3诱导细胞凋亡，这表明凋亡机制可能参与子宫内海洛因暴露所致的神经行为致畸性。
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