粉防己碱对白血病多药耐药细胞株K562/A02中核因子-κB表达的影响

[Effects of tetrandrine on nuclear factor-kappaB expression in leukemia multidrug-resistant cell line K562/A02].

作者信息

Chen Bao-An, Su Ai-Ling, Cheng Jian, Zhao Hui-Hui, Li Guo-Hong, Wang Xue-Mei

机构信息

Department of Hematology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu Province 210009, China.

出版信息

Zhong Xi Yi Jie He Xue Bao. 2008 Sep;6(9):956-9. doi: 10.3736/jcim20080916.

DOI:10.3736/jcim20080916

PMID:18782542

Abstract

OBJECTIVE

To investigate the effects of tetrandrine (Tet) on nuclear factor kappaB (NF-kappaB) expression in leukemia cell line K562 and multidrug-resistant K562/A02 cell line.

METHODS

The activations of NF-kappaB in K562 and K562/A02 cells and the effects of 1 micromol/L Tet on NF-kappaB expression were determined by immunocytochemistry and Western blot assay.

RESULTS

Tet had no effect on NF-kappaB expression in K562 cells after 6- and 12-hour treatment (P>0.05), and K562/A02 cells displayed higher level of NF-kappaB protein expression than their parental K562 cells (P<0.01). Tet could significantly down-regulate NF-kappaB protein expression and nuclear translocation in K562/A02 cells shown by immunocytochemistry and Western blot, and this decrease became more significant after 12-hour treatment than after at 6-hour treatment (P<0.05).

CONCLUSION

Activation of NF-kappaB may be related to multidrug resistance of K562/A02 cell line. And the inhibition of NF-kappaB activation by Tet leads to multidrug resistance reversal in K562/A02 cell line.

摘要

目的

研究粉防己碱（Tet）对白血病细胞株K562及多药耐药K562/A02细胞株中核因子κB（NF-κB）表达的影响。

方法

采用免疫细胞化学和蛋白质印迹法检测K562和K562/A02细胞中NF-κB的激活情况以及1 μmol/L Tet对NF-κB表达的影响。

结果

处理6小时和12小时后，Tet对K562细胞中NF-κB的表达无影响（P>0.05），且K562/A02细胞中NF-κB蛋白表达水平高于其亲代K562细胞（P<0.01）。免疫细胞化学和蛋白质印迹结果显示，Tet可显著下调K562/A02细胞中NF-κB蛋白的表达及核转位，且处理12小时后的降低程度比处理6小时后更显著（P<0.05）。