Kolenko Petr, Dohnálek Jan, Dusková Jarmila, Skálová Tereza, Collard Renata, Hasek Jindrich
Department of Structure Analysis, Institute of Macromolecular Chemistry AS CR, Praha, Czech Republic.
Immunology. 2009 Mar;126(3):378-85. doi: 10.1111/j.1365-2567.2008.02904.x. Epub 2008 Sep 6.
The structure of the Fc fragment of monoclonal antibody IgG2b from hybridom M75 of Mus musculus has been determined by single crystal X-ray diffraction. This is the first report of the structure of the murine immunoglobulin isotype IgG2b. The structure refined at 2.1 A resolution provides more detailed structural information about native oligosaccharides than was previously available. High-quality Fourier maps provide a clear identification of alpha-l-fucose with partial occupancy in the first branch of the antennary oligosaccharides. A unique Fc:Fc interaction was observed at the C(H)2-C(H)3 interface.
小家鼠杂交瘤M75产生的单克隆抗体IgG2b的Fc片段结构已通过单晶X射线衍射确定。这是小鼠免疫球蛋白同种型IgG2b结构的首次报道。在2.1埃分辨率下精修的结构提供了比以前更多关于天然寡糖的详细结构信息。高质量的傅里叶图清晰地识别出在触角状寡糖的第一个分支中部分占据的α-L-岩藻糖。在C(H)2-C(H)3界面观察到独特的Fc:Fc相互作用。