Kibangou I B, Bureau F, Allouche S, Arhan P, Bouglé D
Laboratoire de Physiologie Digestive et Nutritionnelle, CHU de Caen, 14033, Caen, France.
Food Chem Toxicol. 2008 Nov;46(11):3414-6. doi: 10.1016/j.fct.2008.08.014. Epub 2008 Aug 20.
EDTA is a well known enhancer of iron absorption; however, the precise way of absorption of iron ingested in presence of EDTA is not known; some data suggest it could use a passive, non regulated paracellular way. Iron (sulphate or gluconate) absorption by Caco-2 cells was assessed in presence of EDTA. EDTA did not change the apical uptake of iron; transport in the basal chamber increased by 98% for FeSO4 and 95% for Fe gluconate. By contrast, intracellular storage decreased by 31% for FeSO4 and 64% for Fe gluconate. In addition EDTA induced a significant increase of permeability of the cell monolayer assessed by a decrease of transepithelial electrical resistance: 314+/-34 Omegacm(-2) to 235+/-57 Omegacm(-2) for sulphate, 414+/-33 Omegacm(-2) to 223+/-36 Omegacm(-2) for gluconate; iron free control: 410+/-10 Omegacm(-2).
These results suggest that in presence of EDTA iron absorption occurs mainly by the paracellular instead of the regulated cellular way, that could potentially enhance its toxicity.
乙二胺四乙酸（EDTA）是一种众所周知的铁吸收增强剂；然而，在EDTA存在的情况下摄入的铁的确切吸收方式尚不清楚；一些数据表明它可能通过被动的、非调节性的细胞旁途径吸收。在EDTA存在的情况下评估了Caco-2细胞对铁（硫酸盐或葡萄糖酸盐）的吸收。EDTA并未改变铁的顶端摄取；对于硫酸亚铁，基底室中的转运增加了98%，对于葡萄糖酸亚铁则增加了95%。相比之下，硫酸亚铁的细胞内储存减少了31%，葡萄糖酸亚铁减少了64%。此外，通过跨上皮电阻的降低评估，EDTA诱导细胞单层的通透性显著增加：对于硫酸盐，从314±34Ωcm⁻²降至235±57Ωcm⁻²，对于葡萄糖酸盐，从414±33Ωcm⁻²降至223±36Ωcm⁻²；无铁对照：410±10Ωcm⁻²。
这些结果表明，在EDTA存在的情况下，铁的吸收主要通过细胞旁途径而非调节性细胞途径发生，这可能会增强其毒性。
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