Tchélidzé Pavel, Chatron-Colliet Aurore, Thiry M, Lalun Natahlie, Bobichon Hélène, Ploton Dominique
Institute of Biology Iv Javakhishvili, Tbilissi State University, Tbilissi, Georgia.
Crit Rev Oncol Hematol. 2009 Feb;69(2):127-43. doi: 10.1016/j.critrevonc.2008.07.022. Epub 2008 Sep 10.
Changes in nuclear structures are widely used by pathologists as diagnostic and prognostic indicators in cancer cells. Recent studies have demonstrated that the cell nucleus is probably the most complex organelle in the cell. It contains the genome and is the site of all related activities such as DNA repair, DNA duplication, RNA synthesis, RNA processing and RNA transport. These activities take place within dynamic three-dimensional compartments. The detailed study of these compartments requires an approach termed "cell tomography" based on 3D imaging using confocal microscopy and electron tomography. In this paper, we will first summarize the most recent findings concerning the organization of the cell nucleus. We will then describe markers used to identify molecules specific for various nuclear compartments and their use in tomography of the cell nucleus by confocal microscopy and electron tomography.
核结构的变化被病理学家广泛用作癌细胞诊断和预后的指标。最近的研究表明，细胞核可能是细胞中最复杂的细胞器。它包含基因组，是所有相关活动的场所，如DNA修复、DNA复制、RNA合成、RNA加工和RNA转运。这些活动发生在动态的三维隔室中。对这些隔室的详细研究需要一种基于共聚焦显微镜和电子断层扫描的3D成像技术，即“细胞断层扫描”。在本文中，我们将首先总结关于细胞核组织的最新发现。然后，我们将描述用于识别各种核隔室特异性分子的标记物，以及它们在共聚焦显微镜和电子断层扫描细胞核断层成像中的应用。
Zotero 插件