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来自喜马拉雅山西部废弃茶园的选定茶树(Camellia sinensis)种质间的高水平遗传多样性。

High level of genetic diversity among the selected accessions of tea (Camellia sinensis) from abandoned tea gardens in western Himalaya.

作者信息

Karthigeyan S, Rajkumar S, Sharma R K, Gulati Ashu, Sud R K, Ahuja P S

机构信息

Institute of Himalayan Bioresource Technology (CSIR), P.O. Box No. 6, Palampur, Himachal Pradesh, 176 061, India.

出版信息

Biochem Genet. 2008 Dec;46(11-12):810-9. doi: 10.1007/s10528-008-9195-1. Epub 2008 Sep 11.

Abstract

To revive cultivation of the tea unique to the western Himalayan region, it is important to evaluate the seed-derived bushes available in the area's abandoned gardens. This study used quantitative leaf characters, catechin content, and AFLP markers to assess these China cultivar type bushes. Compared with other China cultivar germplasm, these accessions showed a higher level of diversity among themselves. Among the quantitative morphological characters, leaf length is important in distinguishing the accessions studied, with a high loading value in the principal component analysis. The catechins and AFLP markers displayed the genetic makeup of the accessions. Other than total catechins, the trihydroxylated catechins showed a high loading value in differentiating the accessions. The genetic control of the ratio of dihydroxylated and trihydroxylated catechins is found to be based on a correlation with AFLP markers. The genetic similarity between Kangra Asha and Kangra Jat suggests that Kangra Jat must be descended from Kangra Asha. Kangra Jat is well adapted to local environmental conditions, as is evident from its high catechin content.

摘要

为恢复喜马拉雅西部地区特有的茶树种植,评估该地区废弃茶园中现有的种子繁殖茶树很重要。本研究使用叶片数量性状、儿茶素含量和扩增片段长度多态性(AFLP)标记来评估这些中国品种类型的茶树。与其他中国品种种质相比,这些种质之间表现出更高水平的多样性。在数量形态性状中,叶长在区分所研究的种质方面很重要,在主成分分析中具有较高的载荷值。儿茶素和AFLP标记展示了种质的遗传构成。除总儿茶素外,三羟基化儿茶素在区分种质方面具有较高的载荷值。发现二羟基化和三羟基化儿茶素比例的遗传控制基于与AFLP标记的相关性。康格拉阿莎(Kangra Asha)和康格拉贾特(Kangra Jat)之间的遗传相似性表明,康格拉贾特必定是康格拉阿莎的后代。康格拉贾特对当地环境条件适应性良好,这从其高儿茶素含量中可见一斑。

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