由聚阳离子诱导的肌动蛋白组装形成导致的脂质体形态发生。

Morphogenesis of liposomes caused by polycation-induced actin assembly formation.

作者信息

Maemichi Haruka, Shikinaka Kazuhiro, Kakugo Akira, Furukawa Hidemitsu, Osada Yoshihito, Gong Jian Ping

机构信息

Department of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.

出版信息

Langmuir. 2008 Oct 21;24(20):11975-81. doi: 10.1021/la802057c. Epub 2008 Sep 12.

DOI:10.1021/la802057c

PMID:18785715

Abstract

We investigated the effects of polycation-mediated actin assembly on the morphological transformation of the lipid vesicle membrane by spatiotemporally controlling actin assembly. By triggering the radical polymerization of the cationic monomer using UV irradiation, we achieved a varied photoinduced assembly of actin in bulk solution. Furthermore, we designed liposomes containing actin and cationic monomers. In these actin-encapsulated liposomes, various actin assemblies were formed by UV irradiation similar to that observed in bulk solution. Moreover, morphogenesis of actin-encapsulated liposomes was observed in liposomes encapsulated with G-actin but not with F-actin. This result indicates that a dynamic polymerization of G-actin is important for vesicle protrusion.

摘要

我们通过时空控制肌动蛋白组装，研究了聚阳离子介导的肌动蛋白组装对脂质囊泡膜形态转变的影响。通过紫外线照射引发阳离子单体的自由基聚合，我们在本体溶液中实现了肌动蛋白不同的光诱导组装。此外，我们设计了含有肌动蛋白和阳离子单体的脂质体。在这些包裹了肌动蛋白的脂质体中，通过紫外线照射形成了各种肌动蛋白组装体，类似于在本体溶液中观察到的情况。此外，在用G-肌动蛋白而非F-肌动蛋白包裹的脂质体中观察到了包裹肌动蛋白的脂质体的形态发生。这一结果表明，G-肌动蛋白的动态聚合对于囊泡突出很重要。