增强绿色荧光蛋白（EGFP）和新霉素磷酸转移酶II（NPTII）蛋白的表达与死亡的转基因克隆牛的器官异常无关。
Expression of EGFP and NPTII protein is not associated with organ abnormalities in deceased transgenic cloned cattle.
作者信息
Liu Yan, Wu Qian, Cui Huiting, Li Qinghe, Zhao Yiqiang, Luo Juan, Liu Qiuyue, Sun Xiuzhu, Tang Bo, Zhang Lei, Dai Yunping, Li Ning
机构信息
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, People Republic of China.
出版信息
Cloning Stem Cells. 2008 Dec;10(4):421-8. doi: 10.1089/clo.2008.0015.
Both enhanced green fluorescence protein (EGFP) and neomycin phosphotransferase type II enzyme (NPTII) are widely used in transgenic studies, but their side effects have not been extensively investigated. In this study, we evaluated the expression profiles of the two marker genes and the relationship between their expression and organ abnormalities. Eight transgenic cloned cattle were studied, four harboring both EGFP and NPTII, and four harboring only the NPTII gene. Four age-matched cloned cattle were used as controls. EGFP and NPTII expression were measured and detected by Q-PCR, Western blot, ELISA, and RIA in heart, liver, and lungs, and the values ranged from 0.3 to 5 microg/g. The expression profiles exhibited differential or mosaic pattern between the organs, the pathologic symptoms of which were identified, but were similar to those of age-matched cloned cattle. All data indicated that the expression of EGFP and NPTII is not associated with organ abnormalities in transgenic cloned cattle.
增强型绿色荧光蛋白（EGFP）和新霉素磷酸转移酶II型（NPTII）在转基因研究中都被广泛应用，但其副作用尚未得到广泛研究。在本研究中，我们评估了这两个标记基因的表达谱以及它们的表达与器官异常之间的关系。研究了8头转基因克隆牛，其中4头同时携带EGFP和NPTII，另外4头只携带NPTII基因。选取4头年龄匹配的克隆牛作为对照。通过Q-PCR、蛋白质免疫印迹法（Western blot）、酶联免疫吸附测定（ELISA）和放射免疫分析（RIA）检测心脏、肝脏和肺中EGFP和NPTII的表达，其值范围为0.3至5微克/克。表达谱在各器官之间呈现出差异或镶嵌模式，确定了其病理症状，但与年龄匹配的克隆牛相似。所有数据表明，EGFP和NPTII的表达与转基因克隆牛的器官异常无关。
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