果蝇胚胎发育过程中的凋亡力与组织动力学
Apoptotic force and tissue dynamics during Drosophila embryogenesis.
作者信息
Toyama Yusuke, Peralta Xomalin G, Wells Adrienne R, Kiehart Daniel P, Edwards Glenn S
机构信息
Physics Department and Free Electron Laser Laboratory, Duke University, Durham, NC 27708, USA.
出版信息
Science. 2008 Sep 19;321(5896):1683-6. doi: 10.1126/science.1157052.
Abstract
Understanding cell morphogenesis during metazoan development requires knowledge of how cells and the extracellular matrix produce and respond to forces. We investigated how apoptosis, which remodels tissue by eliminating supernumerary cells, also contributes forces to a tissue (the amnioserosa) that promotes cell-sheet fusion (dorsal closure) in the Drosophila embryo. We showed that expression in the amnioserosa of proteins that suppress or enhance apoptosis slows or speeds dorsal closure, respectively. These changes correlate with the forces produced by the amnioserosa and the rate of seam formation between the cell sheets (zipping), key processes that contribute to closure. This apoptotic force is used by the embryo to drive cell-sheet movements during development, a role not classically attributed to apoptosis.
摘要
了解后生动物发育过程中的细胞形态发生需要知道细胞和细胞外基质如何产生并响应力。我们研究了细胞凋亡如何通过消除多余细胞来重塑组织，同时也为促进果蝇胚胎中细胞层融合（背侧闭合）的组织（羊膜浆膜）贡献力量。我们发现，在羊膜浆膜中表达抑制或增强细胞凋亡的蛋白质分别会减缓或加速背侧闭合。这些变化与羊膜浆膜产生的力以及细胞层之间形成接缝（拉链化）的速率相关，而这些是促成闭合的关键过程。胚胎利用这种凋亡力在发育过程中驱动细胞层运动，这一作用并非传统上认为的细胞凋亡所具有的。
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本文引用的文献
1
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2
Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development.果蝇发育过程中背侧闭合过程中力和形态发生不对称性的上调。
Biophys J. 2007 Apr 1;92(7):2583-96. doi: 10.1529/biophysj.106.094110. Epub 2007 Jan 11.
3
Two distinct modes of myosin assembly and dynamics during epithelial wound closure.上皮伤口愈合过程中肌球蛋白组装和动力学的两种不同模式。
J Cell Biol. 2007 Jan 1;176(1):27-33. doi: 10.1083/jcb.200609116.
4
Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis.果蝇变态发育过程中指导上皮细胞层替换的外在和内在机制。
Development. 2007 Jan;134(2):367-79. doi: 10.1242/dev.02728. Epub 2006 Dec 13.
5
Multicellular rosette formation links planar cell polarity to tissue morphogenesis.多细胞玫瑰花结的形成将平面细胞极性与组织形态发生联系起来。
Dev Cell. 2006 Oct;11(4):459-70. doi: 10.1016/j.devcel.2006.09.007.
6
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7
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8
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9
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10
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