Seo Seung-kee, Wei Alexander
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907-2084, USA.
Org Biomol Chem. 2008 Sep 21;6(18):3362-5. doi: 10.1039/b810158b. Epub 2008 Jul 25.
L-(-)-sucrose was efficiently synthesized using intramolecular aglycon delivery and used to elucidate osmotic effects on the activity of invertase, which catalyzes the hydrolysis of D-(+)-sucrose. The osmotic effect imposed by L-sucrose was responsible for more than 30% of the activity loss ascribed otherwise to "substrate inhibition."
通过分子内苷元传递高效合成了L-(-)-蔗糖,并用于阐明渗透压对转化酶活性的影响,该酶催化D-(+)-蔗糖的水解。L-蔗糖产生的渗透压导致了超过30%的活性损失,否则这些损失会归因于“底物抑制”。