Zheng Pu, Zhou Wei, Ni Ye, Jiang Min, Wei Ping, Sun Zhihao
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School ofBiotechnology, Jiangnan University, Wuxi 214122, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Jun;24(6):1051-5.
Actinobacillus succinogenes is a promising candidate for the production of bio-based succinic acid. Previously, we isolated a succinic acid-producing strain Actinobacillus succinogenes CGMCC 1593 from bovine rumen. In this paper, the influence of the environmental factors such as gas phase, pH, ORP, on succinic acid production by A. succinogenes CGMCC 1593 was studied. The results showed that CO2 was the optimum gas phase for anaerobic fermentation ofA. succinogenes CGMCC 1593 as well as one of the substrate for the succinic acid synthesis. Using MgCO3 as a pH regulator, the pH was maintained within 7.1-6.2 during the anaerobic fermentation for the cell growth and acid production of A. succinogenes CGMCC 1593. Our results showed that low initial ORP was disadvantageous for the growth of A. succinogenes CGMCC 1593 and an ORP of -270 mV was demonstrated to be beneficial to the succinic acid production. By adding Na2S.9H2O to decrease ORP to -270 mV at the end of exponential growth phase in batch culture of A. succinogenes CGMCC 1593, the succinic acid concentration reached 37 g/L and the yield of succinic acid was 129% at 48 h. This work might provide valuable information for further optimization of succinic acid fermentation by A. succinogenes CGMCC 1593.
产琥珀酸放线杆菌是生产生物基琥珀酸的一个有前景的候选菌株。此前，我们从牛瘤胃中分离出了一株产琥珀酸的菌株——产琥珀酸放线杆菌CGMCC 1593。本文研究了气相、pH值、氧化还原电位等环境因素对产琥珀酸放线杆菌CGMCC 1593产琥珀酸的影响。结果表明，CO2是产琥珀酸放线杆菌CGMCC 1593厌氧发酵的最佳气相，也是琥珀酸合成的底物之一。以碳酸镁作为pH调节剂，在厌氧发酵过程中，产琥珀酸放线杆菌CGMCC 1593的细胞生长和产酸阶段，pH值维持在7.1 - 6.2之间。我们的结果表明，较低的初始氧化还原电位不利于产琥珀酸放线杆菌CGMCC 1593的生长，而-270 mV的氧化还原电位被证明有利于琥珀酸的产生。在产琥珀酸放线杆菌CGMCC 1593分批培养的指数生长期结束时，通过添加Na2S·9H2O将氧化还原电位降至-270 mV，48 h时琥珀酸浓度达到37 g/L，琥珀酸产量为129%。这项工作可能为进一步优化产琥珀酸放线杆菌CGMCC 1593的琥珀酸发酵提供有价值的信息。
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