Yagati Ajay Kumar, Kim Sang-Uk, Min Junhong, Choi Jeong-Woo
Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
Biosens Bioelectron. 2009 Jan 1;24(5):1503-7. doi: 10.1016/j.bios.2008.07.080. Epub 2008 Aug 19.
In this study a protein-based multi-bit biomemory device consisting of recombinant azurin with its cysteine residue modified by site-directed mutagenesis method has been developed. The recombinant azurin was directly immobilized on four different gold (Au) electrodes patterned on a single silicon substrate. Using cyclic voltammetry (CV), chronoamperometry (CA) and open circuit potential amperometry (OCPA) methods the memory function of the fabricated biodevice was validated. The charge transfer occurs between protein molecules and Au electrode enables a bi-stable electrical conductivity allowing the system to be used as a digital memory device. Data storage is achieved by applying redox potentials which are within the range of 200mV. Oxidation and open circuit potentials with current sensing were used for writing and reading operations respectively. Applying oxidation potentials in different combinations to each Au electrodes, multi-bit information was stored in to the azurin molecules. Finally, the switching robustness and reliability of the proposed device has been examined. The results suggest that the proposed device has a function of memory and can be used for the construction of nano-scale multi-bit information storage device.
在本研究中，已开发出一种基于蛋白质的多位生物记忆器件，该器件由通过定点诱变方法修饰其半胱氨酸残基的重组天青蛋白组成。重组天青蛋白直接固定在单个硅基板上图案化的四个不同金（Au）电极上。使用循环伏安法（CV）、计时电流法（CA）和开路电位电流法（OCPA）对所制备生物器件的记忆功能进行了验证。蛋白质分子与金电极之间发生的电荷转移实现了双稳态电导率，从而使该系统能够用作数字记忆器件。通过施加200mV范围内的氧化还原电位来实现数据存储。氧化电位和带电流传感的开路电位分别用于写入和读取操作。通过向每个金电极施加不同组合的氧化电位，多位信息被存储到天青蛋白分子中。最后，对所提出器件的开关稳健性和可靠性进行了研究。结果表明，所提出的器件具有记忆功能，可用于构建纳米级多位信息存储器件。
