使用氟喹诺酮1和19F核磁共振波谱法测定体内肝细胞内pH值。

Hepatic intracellular pH in vivo using F-quene 1 and 19F NMR spectroscopy.

作者信息

Beech J S, Iles R A

机构信息

Medical Unit (Cellular Mechanisms Research Group), London Hospital Medical College, Whitechapel.

出版信息

Magn Reson Med. 1991 Jun;19(2):386-92. doi: 10.1002/mrm.1910190229.

DOI:10.1002/mrm.1910190229

PMID:1881327

Abstract

Intracellular pH (pHi) has been determined in vivo in livers from anaesthetized rats using both 31P NMR and 19F NMR spectroscopy. In the 31P NMR study pHi, determined from the chemical shift of endogenous Pi, was found to be 7.26 +/- 0.02. In the 19F NMR study on a separate group of animals pHi was determined from the chemical shift of F-Quene 1 infused via the portal vein and found to be 7.18 +/- 0.01. In approximately half of the 19F NMR studies no 19F signal could be detected in the liver probably because of the two probes could be caused by different intra- or intercellular distributions or by unknown effects of F-Quene 1 on metabolism.

摘要

利用31P核磁共振和19F核磁共振光谱法，已在麻醉大鼠的肝脏中对细胞内pH值（pHi）进行了体内测定。在31P核磁共振研究中，根据内源性磷酸（Pi）的化学位移确定的pHi为7.26±0.02。在对另一组动物进行的19F核磁共振研究中，pHi是根据经门静脉注入的F-奎宁1的化学位移确定的，结果为7.18±0.01。在大约一半的19F核磁共振研究中，肝脏中未检测到19F信号，这可能是因为两种探针在细胞内或细胞间的分布不同，或者是由于F-奎宁1对代谢的未知影响所致。