Hartmann Annetta, Patz Claus-Dieter, Andlauer Wilfried, Dietrich Helmut, Ludwig Michael
Institute of Life Technologies, University of Applied Sciences Valais, CH-1950 Sion, Switzerland.
J Agric Food Chem. 2008 Oct 22;56(20):9484-9. doi: 10.1021/jf801555q. Epub 2008 Sep 27.
To determine the effects of different processing steps, such as enzymatic treatment of the mash and pasteurization, on selected quality parameters, strawberries were processed to juices and purees. To identify the processing steps causing the highest losses, samples were taken after each step, and ascorbic acid, total phenols, anthocyanins, and antioxidant capacity were analyzed. To assess the antioxidant capacity, three different methods were applied: the trolox equivalent antioxidant capacity (TEAC), the ferric reducing antioxidant power (FRAP), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, showing correlation coefficients of 0.889 to 0.948. The antioxidant capacity decreased with processing steps except heat treatment, which partly caused an increase due to the formation of antioxidant active products. The content of ascorbic acid, in comparison to that in the frozen strawberries, decreased significantly during the processing of the fruit to puree by 77%. In the pressed cloudy juices, the loss of ascorbic acid was 37%. The decline of phenolic compounds, measured as total polyphenols and anthocyanins, was smaller (between 30-40%). Pressing and pasteurization were the most critical steps for the decrease of these compounds. The enzymatic treatment of the mash within 90 min supported the release of secondary plant metabolites, while ascorbic acid is reduced up to 20%.
为了确定不同加工步骤(如对果泥进行酶处理和巴氏杀菌)对选定质量参数的影响,将草莓加工成果汁和果泥。为了确定导致损失最大的加工步骤,在每个步骤之后采集样品,并分析抗坏血酸、总酚、花青素和抗氧化能力。为了评估抗氧化能力,应用了三种不同的方法:特洛克斯等效抗氧化能力(TEAC)、铁还原抗氧化能力(FRAP)和2,2-二苯基-1-苦基肼(DPPH)测定法,相关系数在0.889至0.948之间。除热处理外,抗氧化能力随加工步骤而降低,热处理由于抗氧化活性产物的形成而部分导致抗氧化能力增加。与冷冻草莓相比,在将水果加工成果泥的过程中,抗坏血酸含量显著下降了77%。在压榨的混浊果汁中,抗坏血酸的损失为37%。以总多酚和花青素衡量的酚类化合物的下降幅度较小(在30%-40%之间)。压榨和巴氏杀菌是这些化合物减少的最关键步骤。在90分钟内对果泥进行酶处理有助于次生植物代谢产物的释放,而抗坏血酸最多可减少20%。