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通过基质辅助激光解吸电离质谱（MALDI MS）分析并经串联质谱（MS/MS）鉴定的肽段假阳性序列匹配率。

The rate of false positive sequence matches of peptides profiled by MALDI MS and identified by MS/MS.

作者信息

Stoop Marcel P, Lamers Robert-Jan A N, Burgers Peter C, Sillevis Smitt Peter A E, Hintzen Rogier Q, Luider Theo M

机构信息

Laboratories of Neuro-Oncology/Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam, The Netherlands.

出版信息

J Proteome Res. 2008 Nov;7(11):4841-7. doi: 10.1021/pr800489a. Epub 2008 Oct 7.

DOI:10.1021/pr800489a
PMID:18837534
Abstract

In most MALDI peptide profiling cases, sequencing is required to identify peptides of interest, preferentially by using different mass spectrometry techniques. Using identical samples, we determined the number of false positive matches in sequence of peptide identification using different mass spectrometers. This paper demonstrates that the reliability of the identification phase greatly benefits from concerted MS-technologies and determines the influence of mass accuracy, signal-to-noise and statistical score on peptide identification.

摘要

在大多数基质辅助激光解吸/电离肽谱分析案例中，需要进行测序来鉴定感兴趣的肽段，优先采用不同的质谱技术。我们使用相同的样品，确定了使用不同质谱仪进行肽段鉴定时序列中假阳性匹配的数量。本文表明，鉴定阶段的可靠性极大地受益于协同的质谱技术，并确定了质量精度、信噪比和统计得分对肽段鉴定的影响。

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