[Expression of CCR3 and CD34 in lung tissues and bone marrow of Guinea pig asthmatic models].

AIM

To explore the mechanism of asthma, by evaluating the changes of CCR3 and EOS expression in the lung tissues and bone marrow of Guinea pig asthmatic models at different times.

METHODS

Guinea pigs (GPs) were sensitized and challenged by OVA to establish the asthmatic model (A, B, C, D, E) . The GPs in control (N) were sensitized and exposed to sterile saline. The sensitized GPs in the models were killed at 30 min, 6 h, 12 h, 24 h and 48 h after challenged by OVA; while the GPs in control were killed at 12 h after saline challenge. The slides were prepared from peripheral blood and bone marrow and then stained with Wright's staining .The total number and percentage of EOS were counted and the expression of CCR3 and CD34 in bone marrow was detected by immunohistochemistry. The pathologic samples of lung tissues were stained with HE. The expression of CCR3 and CD34 of lung tissues was detected by immunohistochemistry.

RESULTS

(1)The expression of CCR3, CD34 and EOS in the bone marrow and peripheral tissue with allergic asthma was significantly higher than that in the controls. (2)After challenged by OVA , the expression of CCR3, CD34 and EOS was as fallows: it was at normal level in 30 min except the rise of CD34 rise in lung tissues, it rose and reached the peak from 6 h to 12 h, except the descent of CD34 in lungs; then it decreased in 24 h and reached normal level after 48 h. (3)The changes of bone marrow were earlier than peripheral tissue; The expression of CCR3 was earlier than the expression of CD34 and the recruitment of EOS.CCR3 and CD34 had linearity correlation with EOS except in lung tissues.

CONCLUSION

There is a passage between bone marrow and lung tissue, through which CD34(+) stem cells and EOS passes The expression of CCR3 makes the quick recruitment of CD34(+) cells and EOS from bone marrow to lung tissue possible.