Gammon M T, Isselbacher K J
J Cell Physiol. 1976 Dec;89(4):759-64. doi: 10.1002/jcp.1040890438.
A variant of polyoma BHK cells is described which is capable of growing in a glutamine-free medium. These glutamine-independent variant cells (GIV) have a size and morphologic appearance intermediate between the nontransformed (BHK) and polyoma transformed (Py6) cell line. In contrast to Py6, the GIV cells grow more slowly, produce less lactic acid and do not grow in suspension. However, their adherence to substrate and release of galactosyltransferase into the medium is comparable to that seen with the parent PyBHK. Most striking is the finding that GIV cells are more readily agglutinated by Concanavalin A and when inoculated into hamsters are much more tumorigenic than the parent Py6 cells.
描述了一种多瘤BHK细胞变体，其能够在无谷氨酰胺培养基中生长。这些不依赖谷氨酰胺的变体细胞（GIV）的大小和形态外观介于未转化的（BHK）和多瘤转化的（Py6）细胞系之间。与Py6相比，GIV细胞生长较慢，产生的乳酸较少，且不能悬浮生长。然而，它们对底物的粘附以及半乳糖基转移酶向培养基中的释放与亲本PyBHK细胞相当。最显著的发现是，GIV细胞更容易被伴刀豆球蛋白A凝集，并且接种到仓鼠体内时比亲本Py6细胞具有更强的致瘤性。
