VILLEE C A
J Gen Physiol. 1948 Mar 20;31(4):337-45. doi: 10.1085/jgp.31.4.337.
The metabolism of the imaginal discs of wild type, miniature, vestigial, and four-jointed varieties of Drosophila was investigated using the Cartesian diver ultramicrorespirometer. Wild type and vestigial wing disc respiration is inhibited by cyanide and azide and thus is mediated by an iron or copper porphyrin system, presumable cytochrome-cytochrome oxidase. Respiration is also inhibited by certain hydroxynaphthoquinones, believed to inactivate some enzyme between cytochromes b and c. The respiration of the vestigial and miniature wing discs is increased to normal by the addition of ascorbic acid and to a lesser extent by p-phenylenediamine and hydroquinone, hence the cytochrome oxidase and cytochrome c systems of vestigial and miniature wing discs are normal and the effects of these genes are on enzymes below cytochrome c in the respiratory chain. The respiratory enzymes of the developing imaginal discs of insects are similar to those of a wide variety of cells from bacteria to mammals. The correlation of these biochemical findings with embryological studies of the discs is discussed.
使用笛卡儿潜水钟型微量呼吸计研究了野生型、微型、残翅型和四关节型果蝇成虫盘的代谢。野生型和残翅型翅盘呼吸受到氰化物和叠氮化物的抑制，因此是由铁或铜卟啉系统介导的，推测是细胞色素 - 细胞色素氧化酶。呼吸也受到某些羟基萘醌的抑制，据信这些羟基萘醌会使细胞色素b和c之间的某些酶失活。通过添加抗坏血酸，残翅型和微型翅盘的呼吸增加到正常水平，对苯二胺和对苯二酚在较小程度上也有此作用，因此残翅型和微型翅盘的细胞色素氧化酶和细胞色素c系统是正常的，这些基因的作用是针对呼吸链中细胞色素c以下的酶。昆虫发育中的成虫盘的呼吸酶与从细菌到哺乳动物的多种细胞的呼吸酶相似。讨论了这些生化发现与成虫盘胚胎学研究的相关性。
