利用辣根过氧化物酶显微注射对活鲤鱼视网膜水平细胞进行的群体研究。

Population study of horizontal cells in live carp retinas using microinjections of horseradish peroxidase.

作者信息

Bustamante S, Gutierrez O C

机构信息

Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago.

出版信息

Vision Res. 1991;31(7-8):1107-18. doi: 10.1016/0042-6989(91)90037-6.

DOI:10.1016/0042-6989(91)90037-6

PMID:1891806

Abstract

In order to randomly sample horizontal cells (HCs) of carp retina, horseradish peroxidase (HRP) was delivered into axon terminals by means of vitreally inserted micropipettes in live fish. Survival time was extended to several hours to permit label diffusion into HC somata. A function measuring the relative complexity of cell contours was defined in order to help classify HCs according to their morphological types. The histogram of measurement from 313 HCs showed three modes fitting normal distributions. The numbers of cells in each group were 267, 36 and 10, respectively, representing a relative population of 85.3% H1s, 11.5% H2s and 3.2% H3s. It is suggested that cone HC somata are extended in a monolayer constituted by predominant type-H1 with interspersed groups of both type-H2 and type-H3 cells.

摘要

为了随机采样鲤鱼视网膜的水平细胞（HCs），在活鱼体内通过玻璃体内插入的微吸管将辣根过氧化物酶（HRP）导入轴突终末。将存活时间延长至数小时，以使标记物扩散到HC胞体中。定义了一个测量细胞轮廓相对复杂性的函数，以帮助根据其形态类型对HCs进行分类。对313个HCs的测量直方图显示出符合正态分布的三个模式。每组中的细胞数量分别为267、36和10，分别代表相对比例为85.3%的H1型、11.5%的H2型和3.2%的H3型。提示锥状HC胞体在由占主导的H1型细胞构成的单层中延伸，其间散布着H2型和H3型细胞群。