二甲基亚砜对山羊卵巢组织的灌注及其与冷冻保存后卵泡活力的关系。
Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation.
作者信息
Luz Valesca B, Santos Regiane R, Pinto Leonardo C, Soares Alison A X, Celestino Juliana J H, Mafezoli Jair, Campello Cláudio C, Figueiredo José R, Rodrigues Ana P R
机构信息
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles, University of Ceará, Fortaleza, Brazil.
出版信息
Fertil Steril. 2009 Apr;91(4 Suppl):1513-5. doi: 10.1016/j.fertnstert.2008.07.1778. Epub 2008 Oct 18.
Ovarian cortical fragments (3 x 3 x 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the efficiency of the cryopreservation procedure.
将卵巢皮质碎片（3×3×1毫米）暴露于不同浓度的二甲基亚砜（DMSO）中，通过高效液相色谱法（HPLC）和传统冷冻保存法进一步分析冷冻保护剂灌注情况。这种简单的灌注测试可以预测冷冻保存程序的效率。
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