Guillo Christelle, Roper Michael G
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA.
Analyst. 2008 Nov;133(11):1481-5. doi: 10.1039/b808735k. Epub 2008 Oct 2.
The biological events occurring in the body are complex and challenging to decode. The expression, production, secretion and interaction of proteins, peptides and small molecules often occur in a fast manner and at low concentrations. Methods used to quantify these events must be rapid, selective, sensitive and robust. In recent years, new variations of affinity methodologies have been developed to facilitate quantitation of these biomolecules. This review will focus on selected affinity techniques that have described multi-analyte measurement, high sensitivity techniques, or the application of new affinity reagents applied to conventional technologies to measure analytes involved in cell communication and biomarkers produced in specific disease states.
体内发生的生物事件复杂且难以解读。蛋白质、肽和小分子的表达、产生、分泌及相互作用通常以快速且低浓度的方式发生。用于量化这些事件的方法必须快速、有选择性、灵敏且稳健。近年来，已开发出亲和方法的新变体以促进对这些生物分子的定量分析。本综述将聚焦于特定的亲和技术，这些技术描述了多分析物测量、高灵敏度技术，或应用于传统技术的新型亲和试剂以测量参与细胞通讯的分析物以及特定疾病状态下产生的生物标志物。
