Georgiev S
Vet Med Nauki. 1975;12(5):37-44.
Cytologic, biochemical and immunoelectrophoretic studies were carried out of amniotic fluids in 100 ewes with normal pregnancy and 40 ewes that had miscarried. Each month of pregnancy a total of 20 and 8 animals of the two groups, respectively were studied. It was found that the biochemical and metabolic processes taking place in the fetus lead to changes in the amniotic fluids altering the pH value, the alkali reserve, the content of potassium, calcium, phosphorus, alkaline phosphatase as well as their bactericidal activity. More characteristic changes linked with pregnancy were observed in the cell composition of the amniotic fluids. With advancing in age the increase in cell count was accompanied (staining with Nile blue sulfate) with a rise of the "orange cell" content. The amniotic fluids of ewes with normal pregnancy were found to contain proteins which precipitated with hyper immune sera against blood serum and kidney, heart, and placenta proteins. In ewes that had miscarried the pH values of the amniotic fluids dropped in the months when abortions took place: 7.36, 7.11, 6.90, 6.80 and 6.90, as against 7.41, 7.36, 7.28, 7.17 and 7.18, respectively. Along with pH the alkali reserve also dropped to 37.9 in the first month and 14.20 in the fifth month. In ewes that had miscarried in the 2nd, 3rd, and 4th month these values were 18.90, 14.90, and 13.80 cu. cm, respectively. In cases of abortions the protein composition of the amniotic fluids showed higher levels of the alfa and beta globulins.
对100只正常妊娠母羊和40只流产母羊的羊水进行了细胞学、生化及免疫电泳研究。在妊娠的每个月，分别对两组中的20只和8只动物进行研究。结果发现，胎儿体内发生的生化和代谢过程会导致羊水发生变化，从而改变pH值、碱储备、钾、钙、磷、碱性磷酸酶的含量及其杀菌活性。在羊水的细胞组成中观察到了与妊娠相关的更具特征性的变化。随着年龄的增长，细胞计数增加（用尼罗蓝硫酸盐染色），同时“橙色细胞”含量上升。发现正常妊娠母羊的羊水含有能与抗血清、肾脏、心脏和胎盘蛋白的超免疫血清沉淀的蛋白质。在流产母羊中，流产月份的羊水pH值下降：分别为7.36、7.11、6.90、6.80和6.90，而正常妊娠母羊相应月份的pH值分别为7.41、7.36、7.28、7.17和7.18。除pH值外，碱储备在第一个月也降至37.9，在第五个月降至14.20。在第二、第三和第四个月流产的母羊中，这些值分别为18.90、14.90和13.80立方厘米。在流产的情况下，羊水的蛋白质组成显示α和β球蛋白水平较高。
