Department of Pharmaceutical Sciences, Idaho State University, Pocatello, ID, USA.
J Microencapsul. 2009 Sep;26(6):556-61. doi: 10.1080/02652040802500655.
The objective of this work was to develop uniformly distributed poly(ethylene glycol) grafted poly(lactide-co-glycolide) (PEG-PLGA) nanoparticles of mean size range approximately 100-200 nm using ethyl acetate as the solvent. In the multiple emulsion solvent evaporation method a high pressure microfluidization process was adopted to produce the W/O/W multiple emulsion. Non-toxic ethyl acetate was used to solubilize PEG-PLGA. The mean size of nanoparticles obtained was less than 180 nm. The particle size and size distribution were dependent on the microfluidization conditions applied. Mean particle size steadily increased from 121 nm at three passes to 172 nm at 20 passes of the microfluidizer, indicating that over-processing may be detrimental to PEG-PLGA nanoparticles prepared using this technique. There was no significant alteration of the PEG-PLGA matrix, as evidenced from the differential scanning calorimetric studies.
这项工作的目的是使用乙酸乙酯作为溶剂，开发平均粒径约为 100-200nm 的均匀分布的聚乙二醇接枝聚（乳酸-共-乙醇酸）（PEG-PLGA）纳米粒子。在复乳溶剂蒸发法中，采用高压微射流工艺制备 W/O/W 复乳。无毒的乙酸乙酯用于溶解 PEG-PLGA。所得到的纳米粒子的平均粒径小于 180nm。纳米粒子的粒径和粒径分布取决于所采用的微射流条件。平均粒径从微射流器通过三次的 121nm 稳定增加到通过 20 次的 172nm，表明过度处理可能对使用该技术制备的 PEG-PLGA 纳米粒子有害。从差示扫描量热研究可以看出，PEG-PLGA 基质没有发生明显变化。
