Kawashima H, Fujiño Y, Mochizuki M
Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Jpn J Ophthalmol. 1991;35(1):51-60.
The immunopathogenic mechanisms of experimental autoimmune uveoretinitis (EAU) induced by interphotoreceptor retinoid-binding protein (IRBP) were studied. Lymph node (LN) cells or spleen cells were collected from donor rats 14 days after the immunization with IRBP. After the 3-day pre-incubation of these cells with either IRBP or Concanavalin A (Con A), the cells were transferred to naive syngeneic rats intraperitoneally. EAU was successfully transferred by LN cells pre-cultured with IRBP, while EAU was poorly transferred by LN cells pre-cultured with Con A. On the other hand, spleen cells pre-cultured with either IRBP or Con A were quite potent to transfer EAU. In addition, the enriched helper/inducer T-cells repeatedly transferred EAU, while the enriched suppressor/cytotoxic T-cells did not. There also existed the simultaneous involvement of pinealitis. The histopathological features of EAU and pinealitis were generally similar to those in actively immunized rats. The immune responses to IRBP in recipients with EAU were mostly cellular (delayed hypersensitivity type skin response and proliferative responses of lymphocytes), while humoral responses (Arthus type skin response and antibody activities) were quite weak in most of the recipients. Thus, it was confirmed that cellular immunity plays a major role in the adoptive transfer of EAU by IRBP as in S-antigen-induced EAU.
研究了由光感受器间类视黄醇结合蛋白（IRBP）诱导的实验性自身免疫性葡萄膜视网膜炎（EAU）的免疫致病机制。在IRBP免疫后14天，从供体大鼠收集淋巴结（LN）细胞或脾细胞。在用IRBP或伴刀豆球蛋白A（Con A）对这些细胞进行3天预孵育后，将细胞腹腔内转移至同基因的未免疫大鼠。用IRBP预培养的LN细胞成功转移了EAU，而用Con A预培养的LN细胞转移EAU的效果较差。另一方面，用IRBP或Con A预培养的脾细胞转移EAU的能力很强。此外，富集的辅助/诱导性T细胞反复转移EAU，而富集的抑制/细胞毒性T细胞则不能。同时还存在松果体炎。EAU和松果体炎的组织病理学特征与主动免疫大鼠的特征大致相似。患有EAU的受体对IRBP的免疫反应大多是细胞性的（迟发型超敏反应型皮肤反应和淋巴细胞增殖反应），而在大多数受体中，体液反应（阿瑟斯型皮肤反应和抗体活性）相当微弱。因此，证实了细胞免疫在IRBP介导的EAU过继转移中起主要作用，如同在S抗原诱导的EAU中一样。
