固相微萃取-高效液相色谱法测定水中邻苯二甲酸二异辛酯
Determination of diethylhexyl phtalate in water by solid phase microextraction coupled to high performance liquid chromatography.
作者信息
Kayali Nour, Tamayo Fernando G, Polo-Díez Luis M
机构信息
Mass Spectrometry Service, Faculty of Chemistry, Complutense University of Madrid, Madrid, Spain.
出版信息
Talanta. 2006 Jul 15;69(5):1095-9. doi: 10.1016/j.talanta.2005.12.016. Epub 2006 Jan 18.
Difficulties detected in the determination of the diethylhexylphthalate (DEHP) at trace levels by gas chromatography-mass spectrometry (GC-MS) using SPME, due to its ubiquitous distribution in the environment has been overcome and a new method for the determination of DEHP in drinking water has been proposed. The method is based on solid phase microextraction (SPME) coupled to high-performance liquid chromatography (HPLC). Detection was carried out spectrophotometrically. Calibration graph was linear in the range 10-110 microg/L with a regression coefficient of r(2)=0.998 and a detection limit of 0.6 microg/L. The relative standard deviation was 5 and 2% (n=4) for chromatographic areas and retention times, respectively. The usefulness of the SPME-HPLC technique was confirmed.
气相色谱-质谱联用（GC-MS）结合固相微萃取（SPME）测定痕量邻苯二甲酸二（2-乙基己基）酯（DEHP）时，由于其在环境中广泛分布，测定存在困难，现已克服该问题，并提出了一种测定饮用水中DEHP的新方法。该方法基于固相微萃取（SPME）与高效液相色谱（HPLC）联用。采用分光光度法进行检测。校准曲线在10-110μg/L范围内呈线性，回归系数r²=0.998，检测限为0.6μg/L。色谱峰面积和保留时间的相对标准偏差分别为5%和2%（n=4）。证实了SPME-HPLC技术的实用性。
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