Babić Sandra, Asperger Danijela, Mutavdzić Dragana, Horvat Alka J M, Kastelan-Macan Marija
Laboratory of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 20, HR-10000 Zagreb, Croatia.
Talanta. 2006 Nov 15;70(4):732-8. doi: 10.1016/j.talanta.2006.07.003. Epub 2006 Aug 22.
This work focuses on the application of SPE-HPLC analysis of important veterinary pharmaceuticals from different classes in highly complex wastewater matrix. The pharmaceutical investigated included three sulfonamides (sulfamethazine, sulfadiazine and sulfaguanidine), a sulfonamide synergist (trimethoprim), a tetracycline (oxytetracycline), a fluoroquinolone (enrofloxacine) and a beta-lactame (penicillin G/procaine). The method involves pre-concentration and clean-up by solid phase extraction (SPE) using Oasis HLB extraction catridges. Final analysis of the selected pharmaceutical compounds was carried out by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD). Recoveries were ranged from 68.3 to 97.9% with relative standard deviation below 8.4%. Only for sulfaguanidine low recovery was obtained. Limits of quantification were in the range 1.5-100mug/L depending on pharmaceutical. The described method was applied to the determination of pharmaceuticals in wastewater samples from pharmaceutical industry.
这项工作聚焦于在高度复杂的废水基质中，采用固相萃取-高效液相色谱法（SPE-HPLC）分析不同种类的重要兽用药物。所研究的药物包括三种磺胺类药物（磺胺二甲嘧啶、磺胺嘧啶和磺胺脒）、一种磺胺增效剂（甲氧苄啶）、一种四环素（土霉素）、一种氟喹诺酮类药物（恩诺沙星）以及一种β-内酰胺类药物（青霉素G/普鲁卡因青霉素）。该方法包括使用Oasis HLB萃取柱通过固相萃取（SPE）进行预浓缩和净化。所选药物化合物的最终分析通过高效液相色谱法（HPLC）结合二极管阵列检测器（DAD）进行。回收率在68.3%至97.9%之间，相对标准偏差低于8.4%。仅磺胺脒的回收率较低。定量限在1.5 - 100μg/L范围内，具体取决于药物种类。所描述的方法应用于制药行业废水样品中药物的测定。
