顺铂与雷帕霉素联合化疗对体外培养的 Hep-2 细胞的作用

[Effect of chemotherapy with cisplatin and rapamycin against Hep-2 cells in vitro].

作者信息

Lei Wen-bin, Jia Tao, Su Zhen-zhong, Wen Wei-ping, Zhu Xiao-lin

机构信息

Institute of Otorhinolaryngology, Hospital of Otorhinolaryngology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2008 Oct;28(10):1838-41.

PMID:18971185

Abstract

OBJECTIVE

To evaluate the effect of combined use of rapamycin and cisplatin in the chemotherapy of Hep-2 cells in vitro.

METHODS

The inhibitory effects of rapamycin and cisplatin, used alone or in combination, on the proliferation of Hep-2 cells were measured with MTT assay and median-effect plot analysis. The cell cycle changes after the treatment were analyzed using flow cytometry and Hoechst 33258 immunofluorescence staining.

RESULTS

The IC50 of rapamycin and cisplatin for inducing growth arrest of Hep-2 cells was 11.03 nmol/L and 8.81 micromol/L, respectively. Rapamycin alone caused cell cycle arrest of the Hep-2 cells in G1 phase. Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hep-2 cells (q > 1.15, King's Formula), causing significantly increased apoptosis ratio and growth inhibition rate of Hep-2 cells.

CONCLUSION

Combined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hep-2 cells.

摘要

目的

评估雷帕霉素和顺铂联合应用对体外培养的 Hep-2 细胞化疗的效果。

方法

采用 MTT 法和中位效应图分析法，测定雷帕霉素和顺铂单独及联合使用对 Hep-2 细胞增殖的抑制作用。使用流式细胞术和 Hoechst 33258 免疫荧光染色分析处理后细胞周期的变化。

结果

雷帕霉素和顺铂诱导 Hep-2 细胞生长停滞的 IC50 分别为 11.03 nmol/L 和 8.81 μmol/L。单独使用雷帕霉素可使 Hep-2 细胞的细胞周期停滞于 G1 期。雷帕霉素和顺铂在 Hep-2 细胞化疗中显示出协同作用（q > 1.15，金氏公式），导致 Hep-2 细胞的凋亡率和生长抑制率显著增加。