Pang T, Gardner I D, Blanden R V
Aust J Exp Biol Med Sci. 1976 Aug;54(4):365-70. doi: 10.1038/icb.1976.36.
Peritoneal exudate cells from mice infected with ectromelia virus were cytotoxic for virus-infected target cells as measured in a 51Cr release assay. Cytotoxic activity seemed to be T cell-dependent as it was largely abolished by treatment with anti-theta serum and complement but was not impaired by macrophage depletion. The kinetics of development of cytotoxicity in the peritoneal cavity lagged behind spleen cytotoxicity by 1-2 days. Peak activity in peritoneal cells was present about 6 days after intravenous infection with virus. These studies suggest that macrophages present in the free peritoneal cell populations of ectromelia-infected mice are not cytotoxic for virus-infected target cells. The effect of macrophages in virus clearance is therefore likely to be due to phagocytic rather than cytotoxic effects.
在51Cr释放试验中检测到，感染痘苗病毒的小鼠腹腔渗出细胞对病毒感染的靶细胞具有细胞毒性。细胞毒性活性似乎依赖于T细胞，因为用抗θ血清和补体处理后，其活性大大降低，但巨噬细胞耗竭并未损害其活性。腹腔中细胞毒性发展的动力学比脾脏细胞毒性滞后1 - 2天。静脉感染病毒后约6天，腹腔细胞中出现峰值活性。这些研究表明，痘苗病毒感染小鼠的游离腹腔细胞群体中的巨噬细胞对病毒感染的靶细胞没有细胞毒性。因此，巨噬细胞在病毒清除中的作用可能是由于吞噬作用而非细胞毒性作用。
